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• Improving the separation
• How to manipulate a separation
• Parameters that affect separation
• Normal-phase liquid chromatography
• Reversed-phase liquid chromatography
  
• Ion exchange chromatography
• Ion pairing (ion interaction) chromatography
• Improving the separation
• Simplifying the separation
• Optimization of isocratic separations
  • > Insight and experience?
  • > Computer programs, pro's and cons
  • > The chromatogram as a sum of peaks
    
  • > Experimental designs
    
  • > Rational trial-and-error approaches.
    
  • > Phase selection diagrams
    
  • >
• Optimisation of gradient elution
• PRACTICAL MOBILE PHASE OPTIMIZATION
• SYSTEM OPTIMIZATION
• OBJECTIVE OF THE SEPARATION
  
  
• RECOMMENDATIONS & references
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