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Influence of temperature
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High temperature and temperature programmed HPLC
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Method development
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Applications
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Advantages and limits of HTLC
- > Suitable columns & instruments
- > Reduction of % organic modifier
- > Speed of analysis increases
- > Theoretical potential of different temperatures
- > Comparison of the real potential of two different temperatures
- > Improvement of polar and basic compounds analysis
- > Improvement in peak shape with temperature
- > Variation of retention and change in selectivity with temperature
- > Faster gradient re-equilibration at high temperatures
- > Conclusions
- > Appendix: Calculations and methods
- > References
