Effect of parameters on chromatogram
Harold McNair
,
Virginia Tech
,
USA
Abstract
How do flow rate, polarity, pH, Sample size, column length, I.D., dp influence your chromatogram? Examples and Q&A.
Level
Basic
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Introduction to HPLC columns
Column packings
Influence of column parameters
Selecting and optimizing the column
Effect of parameters on chromatogram
> Flow rate
> Polarity of mobile phase
> Effect of pH
> Sample size
> Column dimensions
> How to increase retention
> How to decrease bandwidth?
Column dimensions and peak broadening
Separation of ionic compounds
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