Effect of parameters on chromatogram
Harold McNair
,
Virginia Tech
,
USA
Abstract
How do flow rate, polarity, pH, Sample size, column length, I.D., dp influence your chromatogram? Examples and Q&A.
Level
Basic
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Introduction to LC
The separation process
The separation process (Animation)
The role of separation techniques
Separation parameters
The resolution equation
The resolution equation (Animation)
eddy diffusion (A-term) (Animation)
Longitudinal diffusion(B-term) (Animation)
Separation & peak overlap
Peak width and peak broadening
The Van Deemter equation
Lee Polite class: Resolution in LC. Video
Lee Polite class: Resolution. Video
Lee Polite class: The resolution tool. Video
Effect of parameters on resolution
Effect of parameters on chromatogram
> Flow rate
> Polarity of mobile phase
> Effect of pH
> Sample size
> Column dimensions
> How to increase retention
> How to decrease bandwidth?
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