Column dimensions and peak broadening
Nico Vonk, Avans+, Breda, The NetherlandsAbstract Band broadening is an important phenomenon in HPLC separations. The band broadening of a chromatographic peak consists of two different and independent contributions: the column and the extra-column or external band broadening processes. Decreasing column dimensions has a number of advantages, but miniaturization puts constraints on maximum sample size and allowable extra-column dead volume. The sample capacity of a column (Qm) is the maximum volume of sample that can be introduced without column overloading and is proportional to column length and the cross sectional area. Smaller columns necessitate lower extra-column dead volume. Detector cell volume is an important extra-column factor.
KeywordsColumn dimensions, Sample capacity (Qm), Column volume, Porosity (å), Asymmetry, Column overloading, Loading capacity, Peak broadening, External broadening, Dead volume, Void volume, Peak volume (Vp), Retention factor, Efficiency, Detector broadening, Particle diameter
LevelBasic
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