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Introduction to LC
- > From open to closed systems
- > The separation process (A)
- > Balancing mobile and stationary phase
- > Adsorption chromatography (LSC)
- > Partition chromatography (LLC)
- > Silica gel basics
- > Chemically bonded phases
- > Polar bonded phases
- > Nonpolar bonded phases (RPLC)
- > Polar bonded vs silica
- > Water can cause problems
- > Fruit juice analysis
- > Non-porous particles
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The separation process
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The separation process (Animation)
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The role of separation techniques
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Separation parameters
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The resolution equation
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The resolution equation (Animation)
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eddy diffusion (A-term) (Animation)
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Longitudinal diffusion(B-term) (Animation)
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Separation & peak overlap
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Peak width and peak broadening
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The Van Deemter equation
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Lee Polite class: Resolution in LC. Video
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Lee Polite class: Resolution. Video
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Lee Polite class: The resolution tool. Video
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Effect of parameters on resolution
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Effect of parameters on chromatogram
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Chromatogram generator
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Test your know-how
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resolution tool sliders
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