Instrumentation for UHPLC

Kenneth Fountain, Waters Corporation, Milford, MA, USA

Abstract This chapter explains the instrumentation needed to take full advantage of smaller particle packed columns, and what is needed to maintain a well functioning system under pressures above 6,000 psi (~400 bar). We also provide guidelines for proper instrumentation and parameter selection to run the best possible separations and to use the optimum columns of a specific dimension and particle size. We will then touch briefly on the effect of unwanted extra-column band spreading on column efficiency and how to minimize it.

LevelBasic

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