More 'bad' LC chromatograms
Nico Vonk
,
Avans+, Breda
,
The Netherlands
Abstract
This chapter shows a series of LC chromatograms with some common problems and their solutions.
Level
Basic
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Troubleshooting LC, basics
How to avoid trouble
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Systematic problem solving
Pressure problems
Peak problems
Resolution problems
Bad column stability
Baseline problems
The pump
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The column
Mobile phase
Detection
Diagnosing 'bad' chromatograms
More 'bad' LC chromatograms
> Ageing?
> Manual valve
> Poor peakshape
> Broad peaks
> Wrong column?
> "Ghost peaks
> Long use
> Restored leak
> Blank baseline
> Wobbly?
> Baseline, "hump" and straight
How to use and regenerate columns
Bad retention time stability
LC troubleshooting advanced course
How to avoid trouble
Diagnosing 'bad' chromatograms
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